Composite

Part:BBa_K4343090:Design

Designed by: huanyu li   Group: iGEM22_NNU-China   (2022-10-09)


pUC-HUH-Scp2-EhElo9(PGAP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1431
    Illegal XhoI site found at 3465
    Illegal XhoI site found at 3498
    Illegal XhoI site found at 7967
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3653
    Illegal NgoMIV site found at 6272
    Illegal NgoMIV site found at 7027
    Illegal AgeI site found at 889
    Illegal AgeI site found at 1543
    Illegal AgeI site found at 1642
    Illegal AgeI site found at 2003
    Illegal AgeI site found at 4706
    Illegal AgeI site found at 5067
    Illegal AgeI site found at 6350
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1546
    Illegal BsaI site found at 2183
    Illegal BsaI site found at 5247
    Illegal BsaI site found at 5784
    Illegal BsaI site found at 6353
    Illegal BsaI site found at 7176
    Illegal BsaI.rc site found at 401
    Illegal BsaI.rc site found at 1540
    Illegal BsaI.rc site found at 6347
    Illegal BsaI.rc site found at 7170
    Illegal BsaI.rc site found at 7440
    Illegal BsaI.rc site found at 8602
    Illegal BsaI.rc site found at 8986
    Illegal SapI.rc site found at 1908
    Illegal SapI.rc site found at 4972


Design Notes

none


Source

from Emiliania huxleyi

References